Western Transfer Buffer (10x) 500mL
Brand: BIOLOGIX
SKU: WTB-510 / WTB-510-1
Out of stock Out of stock
£15.00 - £52.00
£15.00
£52.00
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Product Details
10X Western Blot Transfer Buffer
Biologix 10X Western Blot Transfer Buffer is a concentrated Tris-glycine solution designed for the transfer of proteins from polyacrylamide gels to membranes during Western blotting workflows. Once transferred, proteins can be detected using antigen-antibody binding for semi-quantitative analysis.
The ready-prepared concentrate reduces buffer preparation time and helps minimise dilution and formulation errors.
Biologix 10X Western Blot Transfer Buffer is a concentrated Tris-glycine solution designed for the transfer of proteins from polyacrylamide gels to membranes during Western blotting workflows. Once transferred, proteins can be detected using antigen-antibody binding for semi-quantitative analysis.
The ready-prepared concentrate reduces buffer preparation time and helps minimise dilution and formulation errors.
Key Features
- 10X concentrated transfer buffer
- Suitable for Western blot protein transfer
- Filtered through a 0.22 µm membrane
- DNase- and RNase-free
- Protease-free
- Pyrogen-free
- Clear, colourless liquid
- Helps improve consistency between experiments
Preparation Instructions
- To prepare 1 L of 1X transfer buffer:
- Measure 100 mL of 10X Transfer Buffer.
- Add 200 mL of anhydrous methanol and mix thoroughly.
- Add distilled or deionised water to a final volume of 1 L.
- Mix well before use.
Composition
Contains 250 mM Tris and 1.92 M glycine.
Final pH after dilution to 1X: 8.3 ± 0.1.
This product is supplied non-sterile.
Final pH after dilution to 1X: 8.3 ± 0.1.
This product is supplied non-sterile.
Storage and Shelf Life
Store at room temperature. Stable for up to two years when stored as recommended.
