HEPES Buffer Solution (1 M, pH 7.3) is a sterile, ready-to-use zwitterionic buffer designed to provide stable pH control for cell culture and other sensitive biological applications. With an effective buffering range of pH 6.8–8.2, HEPES maintains consistent pH independently of CO₂ concentration, making it ideal for open culture systems, prolonged cell observation, and applications outside a CO₂ incubator. Chemically and enzymatically stable, it has minimal effects on cellular processes and is supplied filter sterilised for immediate use, with no additional sterilisation required.
Key Features- Stable pH buffering across pH 6.8–8.2, ideal for cell culture applications
- CO₂-independent buffering, suitable for open cultures and extended cell observation
- Chemically and enzymatically stable with minimal interference in biological processes
- Low UV and visible light absorbance, making it suitable for optical applications
- Filter sterilised and low endotoxin, ready to use straight from the bottle
- Recommended working concentration: 10–25 mM